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diaphot 300 inverted microscope  (Nikon)


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    Structured Review

    Nikon diaphot 300 inverted microscope
    Diaphot 300 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diaphot+300+inverted+microscope/Objectives/pm41569431-58-12-11
    Average 99 stars, based on 59676 article reviews
    diaphot 300 inverted microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Inverted Microscopy:

    Article Title: Microscopic nucleic acid-to-protein ratio: A label-free approach for pancreatic cancer detection.
    Article Snippet: .. A Nikon Diaphot 300 inverted microscope with brightfield, phase contrast, and epi-fluorescent imaging capabilities was converted for UV imaging. .. Illumination was provided by a 30 W deuterium lamp (Hamamatsu L11798) delivering full-spectrum output, including 200–400 nm for UV.

    Article Title: The progression of triple-negative breast cancer is linked to a gradual decrease of the BK channel in the plasma membrane and an increase in intracellular structures.
    Article Snippet: 1 Centro Universitario de Investigaciones Biomédicas (CUIB), Universidad de Colima, Colima, México 2 Departamento de Fisiología y Biofísica, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México 3 Secretaría de Ciencia, Humanidades, Tecnología e Innovación (SECIHTI), Universidad de Colima, Colima, México Abstract Big Potassium (BK) channels are gated by both the membrane potential and intracellular Ca2+, and hence they have multiple physiological roles, from canonical control of cellular excitability to modulate cell metabolism, proliferation, and gene expression.. Growing evidence implicates BK channels in several oncogenic processes, including breast cancer.. Though, the role of these channels in the deleterious triple-negative breast cancer (TNBC) is unknown.

    Article Title: Intestinal cell diversity and treatment responses in a parasitic nematode at single cell resolution
    Article Snippet: .. It was then viewed using a Nikon Diaphot 300 inverted microscope equipped with epifluorescence capabilities (UV-2 A filter (blue) and photographed with a Nikon D5100 digital camera. ..

    Article Title: Incubating frozen-thawed buffalo sperm with olive fruit extracts counteracts thawing-induced oxidative stress and improves semen quality.
    Article Snippet: .. At the end of gamete co-incubation, the presumptive zygotes were vortexed for 2 min to remove cumulus cells, and after zona removal by protease (2 mg/mL) digestion, fixed with 60 % methanol in PBS for 30 min, stained with 2.5 μg/mL Hoechst 33342 for 15 min, and mounted on glass slides for subsequent evaluation of nuclear status using a Nikon Diaphot 300 inverted microscope equipped with fluorescence filters. ..

    Article Title: CRISPR-Cas9 HDR Optimization: RAD52, Denatured and 5’-Modified DNA Templates in Knock-In Mice Generation
    Article Snippet: .. Cytoplasmic microinjections were conducted in M2 media using the Transjector 5246 (Eppendorf) and Narishige NT-88NE micromanipulators, mounted on a Nikon Diaphot 300 inverted microscope. ..

    Article Title: TAM-associated CASQ1 mutants diminish intracellular Ca 2+ content and interfere with regulation of SOCE.
    Article Snippet: Total releasable Ca2+ store content was indirectly determined, by measuring cytosolic Ca2+ release induced by treatment with depletion cocktail (ionomycin-CPA-EGTA; ICE) composed by 10 μM ionomycin (ThermoFisher Scientific) and 30 μM cyclopiazonic acid (CPA) in calcium free Ringer solution, containing 0.2 mM EGTA (Hanna et al. 2021; Michelucci et al. 2022). .. Ca2+ measurements were performed using a Nikon Diaphot 300 inverted microscope (Nikon) equipped with a CCD camera (Photometrics, Roper Scientific) and a MetaFluor imaging system (Universal Imaging). .. Fluorescence images were collected with a fluor 20X objective (Nikon).

    Article Title: TAM-associated CASQ1 mutants diminish intracellular Ca 2+ content and interfere with regulation of SOCE
    Article Snippet: Total releasable Ca 2+ store content was indirectly determined, by measuring cytosolic Ca 2+ release induced by treatment with depletion cocktail (ionomycin-CPA-EGTA; ICE) composed by 10 μM ionomycin (ThermoFisher Scientific) and 30 μM cyclopiazonic acid (CPA) in calcium free Ringer solution, containing 0.2 mM EGTA (Hanna et al. ; Michelucci et al. ). .. Ca 2+ measurements were performed using a Nikon Diaphot 300 inverted microscope (Nikon) equipped with a CCD camera (Photometrics, Roper Scientific) and a MetaFluor imaging system (Universal Imaging). .. Fluorescence images were collected with a fluor 20X objective (Nikon).

    Article Title: The epithelial polarity axis controls the resting membrane potential and Cl − co-transport in breast glandular structures
    Article Snippet: .. Fluorescence images were recorded with an inverted IX70 microscope (Olympus) equipped with a Retiga 1300 camera (QImaging, Surrey, BC, Canada) and 40×, NA 1.4 fluor lens, a laser scanning MRC-1024 UV (Bio-Rad) linked to a Diaphot 300 inverted microscope (Nikon, Tokyo, Japan) and oil immersion 60×, numerical aperture (NA) 1.4 apochromatic and 40×, NA 1.4 fluor lenses, and a multiphoton laser scanning confocal Radiance 2100 MP (Bio-Rad Laboratories, Hemel Hampstead, UK) linked to a TE2000 (Nikon, Tokyo, Japan) inverted microscope, with oil immersion 60×, numerical aperture 1.4 apochromatic lens. .. Brightfield images were taken either with the IX70 microscope or a Nikon Diaphot 300 equipped with a QImaging color Retiga 1300 i FAST.

    Imaging:

    Article Title: Microscopic nucleic acid-to-protein ratio: A label-free approach for pancreatic cancer detection.
    Article Snippet: .. A Nikon Diaphot 300 inverted microscope with brightfield, phase contrast, and epi-fluorescent imaging capabilities was converted for UV imaging. .. Illumination was provided by a 30 W deuterium lamp (Hamamatsu L11798) delivering full-spectrum output, including 200–400 nm for UV.

    Article Title: TAM-associated CASQ1 mutants diminish intracellular Ca 2+ content and interfere with regulation of SOCE.
    Article Snippet: Total releasable Ca2+ store content was indirectly determined, by measuring cytosolic Ca2+ release induced by treatment with depletion cocktail (ionomycin-CPA-EGTA; ICE) composed by 10 μM ionomycin (ThermoFisher Scientific) and 30 μM cyclopiazonic acid (CPA) in calcium free Ringer solution, containing 0.2 mM EGTA (Hanna et al. 2021; Michelucci et al. 2022). .. Ca2+ measurements were performed using a Nikon Diaphot 300 inverted microscope (Nikon) equipped with a CCD camera (Photometrics, Roper Scientific) and a MetaFluor imaging system (Universal Imaging). .. Fluorescence images were collected with a fluor 20X objective (Nikon).

    Article Title: TAM-associated CASQ1 mutants diminish intracellular Ca 2+ content and interfere with regulation of SOCE
    Article Snippet: Total releasable Ca 2+ store content was indirectly determined, by measuring cytosolic Ca 2+ release induced by treatment with depletion cocktail (ionomycin-CPA-EGTA; ICE) composed by 10 μM ionomycin (ThermoFisher Scientific) and 30 μM cyclopiazonic acid (CPA) in calcium free Ringer solution, containing 0.2 mM EGTA (Hanna et al. ; Michelucci et al. ). .. Ca 2+ measurements were performed using a Nikon Diaphot 300 inverted microscope (Nikon) equipped with a CCD camera (Photometrics, Roper Scientific) and a MetaFluor imaging system (Universal Imaging). .. Fluorescence images were collected with a fluor 20X objective (Nikon).

    Staining:

    Article Title: Incubating frozen-thawed buffalo sperm with olive fruit extracts counteracts thawing-induced oxidative stress and improves semen quality.
    Article Snippet: .. At the end of gamete co-incubation, the presumptive zygotes were vortexed for 2 min to remove cumulus cells, and after zona removal by protease (2 mg/mL) digestion, fixed with 60 % methanol in PBS for 30 min, stained with 2.5 μg/mL Hoechst 33342 for 15 min, and mounted on glass slides for subsequent evaluation of nuclear status using a Nikon Diaphot 300 inverted microscope equipped with fluorescence filters. ..

    Fluorescence:

    Article Title: Incubating frozen-thawed buffalo sperm with olive fruit extracts counteracts thawing-induced oxidative stress and improves semen quality.
    Article Snippet: .. At the end of gamete co-incubation, the presumptive zygotes were vortexed for 2 min to remove cumulus cells, and after zona removal by protease (2 mg/mL) digestion, fixed with 60 % methanol in PBS for 30 min, stained with 2.5 μg/mL Hoechst 33342 for 15 min, and mounted on glass slides for subsequent evaluation of nuclear status using a Nikon Diaphot 300 inverted microscope equipped with fluorescence filters. ..

    Article Title: The epithelial polarity axis controls the resting membrane potential and Cl − co-transport in breast glandular structures
    Article Snippet: .. Fluorescence images were recorded with an inverted IX70 microscope (Olympus) equipped with a Retiga 1300 camera (QImaging, Surrey, BC, Canada) and 40×, NA 1.4 fluor lens, a laser scanning MRC-1024 UV (Bio-Rad) linked to a Diaphot 300 inverted microscope (Nikon, Tokyo, Japan) and oil immersion 60×, numerical aperture (NA) 1.4 apochromatic and 40×, NA 1.4 fluor lenses, and a multiphoton laser scanning confocal Radiance 2100 MP (Bio-Rad Laboratories, Hemel Hampstead, UK) linked to a TE2000 (Nikon, Tokyo, Japan) inverted microscope, with oil immersion 60×, numerical aperture 1.4 apochromatic lens. .. Brightfield images were taken either with the IX70 microscope or a Nikon Diaphot 300 equipped with a QImaging color Retiga 1300 i FAST.

    Microscopy:

    Article Title: The epithelial polarity axis controls the resting membrane potential and Cl − co-transport in breast glandular structures
    Article Snippet: .. Fluorescence images were recorded with an inverted IX70 microscope (Olympus) equipped with a Retiga 1300 camera (QImaging, Surrey, BC, Canada) and 40×, NA 1.4 fluor lens, a laser scanning MRC-1024 UV (Bio-Rad) linked to a Diaphot 300 inverted microscope (Nikon, Tokyo, Japan) and oil immersion 60×, numerical aperture (NA) 1.4 apochromatic and 40×, NA 1.4 fluor lenses, and a multiphoton laser scanning confocal Radiance 2100 MP (Bio-Rad Laboratories, Hemel Hampstead, UK) linked to a TE2000 (Nikon, Tokyo, Japan) inverted microscope, with oil immersion 60×, numerical aperture 1.4 apochromatic lens. .. Brightfield images were taken either with the IX70 microscope or a Nikon Diaphot 300 equipped with a QImaging color Retiga 1300 i FAST.



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